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CytoTRACK SPAchip® pH Dual detection kit

CytoTRACK SPAchip® pH Dual detection kit

Ref. D-001-PHGR

CytoTRACK SPAchip® pH Dual Detection Kit is the combination of the two technologies addressed to measure intracellular pH: CytoCHECK SPAchip® pH red single-detection andCytoCHECK SPAchip® pH green single-detection.

The product combines these two pH detection technologies so that each of them is internalized in one cell line for co-culturing applications. While tracking the two different cell subtypes and their interaction, measurements of intracellular pH levels by changes in fluorescence intensity are feasible at real time.

The advantages of this product are the combination of our two CytoCHECK pH detection kits, where the selection of the SPAchip® to the corresponding cell line will depend on the features of each. Red emission SPAchip® is recommended for applications which need to overcome high green autofluorescence signals and increase SNR. Green emission SPAchip® advantages are its easiness due to the possibility of using common GFP fluorescence filters and its easy linear response.

Kit box contains:

2 ASSAY SPAchip® eppendorf tube (Quantity: 2.5 x10^6 ASSAY SPAchip®)
1 Control SPAchip® eppendorf tube (Quantity: 5 x10^5 CONTROL SPAchip®)
1 buffer (Quantity: 8mL)
Quick start guide


User protocol

Key features

  • Non-toxic for living single cells.
  • Measures intracellular pH levels by changes in fluorescence intensity.
  • Allows long-term monitoring of intracellular pH changes​
  • Composed of fluorescently labeled silicon microparticles that can be internalized in the cytosol of cultured cells.
  • Provides a more comprehensive study of single-cell physiology and metabolism.
  • Maximizes the performance of most imaging analyzers.
  • Cell type flexibility, no lower limits.
  • Ready-to-use, robust workflow

Technical specifications

Amount~2.5 millions of SPAchips
ApplicationsCell Viability, proliferation, cell image acquisition
Assay Time30 minutes
Assay typeLiving single-cell based
Detection methodRed FluorescenceGreen Fluorescence
Fluorescenceλex: 546 nm; λem: 610 and 707 nmλex:488 nm; λem: 520 nm
For research use onlyNot for use in diagnostic procedures
Incubation timeOvernight
Measuring rangepH 4.5 – 9.0pH 4.5 – 7.5
PlatformFluorescence microscopy, HCS/HCA platforms and flow cytometry
Sample typeAdherent cells, suspension cells
Shelf Life6 months upon arrival
ShippingEnvironmental conditions
SolubilitySoluble in assay buffer (aqueous)
StateSolid-phase film with embedded SPAchips
Storage ConditionsRefrigerate upon arrival (2ºC to 8ºC) and protect from light

Imaging and fluorescence signal analysis

SPAchip® kits are designed to dynamically shift fluorescence intensity values in response to intracellular changes in analyte concentration. Fluorescence intensity is measured by exciting the live cell sample at 488 nm and detecting fluorescence emission at 520 nm. Relative intensity units depend on the instrumental setup (excitation power, detector efficiency, etc). To obtain quantitative data, a calibration curve needs to be analyzed in the same run. Data acquisition can be adapted to fluorescence microscopy and flow cytometry. Additional detection channels, such as brightfield, phase contrast, FSC or SSC, are suggested to better identify individual SPAchips.

Like an eye
in the cell!

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