SPAchip® Technology Concept

SPAchip® is the perfect tool for cell sensing
by dynamic film about cell feeling

Welcome to the Future of Live Cell Imaging!

One of the main technological challenges we face today is to understand the critical role of cellular metabolism in disease. New tools must be designed to simplify and improve cell studies and to get a better understanding of diseases behavior.

Imagine a lab inside a living single cell capable of performing any analysis instantly and over time. SPAchip® technology makes this possible.

What is SPAchip® Technology?

SPAchip® (Suspended-Planar Array chip) technology arises from the fusion of cellular biology and microelectronics fields to monitor cellular processes in real time and to create a dynamic film about cell sensing.

SPAchips are intracellular silicon microchips functionalized with several biomolecules (fluorescence probes, proteins, peptides, and other biomolecules) designed to monitor any cellular pathway in real time without inducing physiological changes or affecting cell viability.

Thanks to a cutting-edge miniaturization process, SPAchips can be easily internalized in living cells and printed with multiple fluorescent probes. By simply changing the SPAchip® fluorescence, you can gain valuable insights into cellular activity.

The first device of its kind

SPAchip® technology stands out as the first intracellular device that can be multiplexed with probes emitting in the same wavelength.

Surface functionalization techniques allow SPAchip® to be printed with nucleic acids, proteins, carbohydrates, lipids, or synthetic chemicals, facilitating the tracking of cell metabolism. Using this revolutionary approach, science will be able to conduct unprecedented living single cell analysis to discover new drugs with a perfect ratio of cost-effectiveness and lower risk.

Real-time monitoring of cellular processes

Try SPAchip® technology today and see
the difference it can make for your research.

SPAchip® Technology Benefits

  • Non-toxic for living single cells.
  • Long-term intracellular measurements by changes in fluorescence intensity.
  • Composed of fluorescently labeled silicon microparticles that can be internalized in the cytosol of cultured cells.
  • Provides a more comprehensive study of single-cell physiology and metabolism.
    More sensitive by locally concentrating the fluorescent probe on the chip surface rather than in solution.
  • Maximizes the performance of most imaging analyzers.
  • Cell type flexibility, no lower limits.
  • An easy to use, robust, and ready-to-use procedure that can be applied immediately.
  • SPAchip® technology workflow fits easily into common existing lab infrastructure and seamlessly integrates with a range of leading platforms, including High-Content Screening (HCS) platforms, confocal microscopes, cell imaging readers, live cell metabolic assay platforms, flow cytometry systems, and more.

SPAchip® as an Early Discovery Tool

Your go/no-go decisions can be informed by high content imaging of your compound interactions with targets.

Using multiplex SPAchip(R) cell-based assays, you can analyze multiple profiles simultaneously in the same sample well. During the testing of safety and the potential toxic effects of the compounds you can predict translational success in vivo by monitoring compounds in complex cellular systems.

Research Areas

The potential uses of SPAchip(R) in drug discovery are at the early stages, in assay development (creating new test systems) and in vitro & in vivo (assessing safety and toxic effects).

Early drug discovery phases are facilitated by SPAchip(R) in several ways:

Phenotypic ScreeningIdentification of Rare RespondersDisease ModelingToxicity AssessmentCellular Signaling PathwaysHigh-Content ScreeningReal-Time KineticsDrug Combination Studies
Allows the observation of living single cellIdentify these rare respondersStudy how individual cells from patients with specific diseases respond to drug candidatesDetecting adverse effects before they become apparent in population-level assaysElucidating complex cellular signaling pathways and their responses to drugsCollecting a wealth of data on individual cell morphology, protein expression, and subcellular localization, providing a comprehensive view of drug effectsAllowing for the tracking of dynamic cellular responses over time.Using to assess the effects of drug combinations on individual cells
Provides insights into how drugs affect different cellsUnderstanding drug resistance or uncovering new therapeutic opportunitiesHelp to develop personalized medicine and more targeted therapiesHaving time and resources in the drug development processHelping in the design of drugs that target specific components of these pathwaysStudying drug kinetics, including drug uptake, metabolismIdentifying synergistic drug combinations and optimizing therapeutic regimens
Better understanding of drug mechanismsLeading to more effective therapies with fewer side effectsInforming the doses, regimens, and drug delivery strategies

Applications and Cell Types

Because each cell is a Storyteller….

The SPAchip® abilities to monitor living single cells during drug discovery offers a deeper understanding of drug responses.
Leads to the identification of new targets and therapeutic opportunities, and can improve the efficiency and safety of the drug development process. It is a powerful tool that has the potential to accelerate drug discovery and improve patient outcomes.

Timelapse of HEK293 cell line (from Human Embryonic Kidney) spheroid formation with internalization of CytoCHECK SPAchip® pH Single-Detection Kit Green. DRAQ5™ in red stained live nuclei. SPAchip® technology allows pH measurement at the different time points of spheroid formation. HEK293 cells were incubated with CytoCHECK SPAchip® pH Single-Detection to allow spheroid formation at 24 A), 28 B), 48 C), and 52 hours D).

A4cell is delighted to present you a disruptive technology able to analyze living single cells
for extended periods offering innovative

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assays with huge advantages:



Connect us to gain from our SPAchip technology!

A Commitment to Advancement
As part of our continuous optimization of our SPAchip® technology, we can offer high value to our customers in terms of High Content Analysis (HCA), High Content Screening (HCS), and cell imaging platform analysis. We are committed to providing the researchers community with the latest advancements in the field of cellular analysis, with the aim of encouraging innovation and ensuring progress in the pharmaceutical and biotechnology industries throughout the world.

Join the SPAchip® Revolution
Get ready to experience the difference SPAchip® technology can make in your routine lab workflow. A versatile, non-toxic tracking technology with multiple analysis possibilities. One wavelength fits all with SPAchip®!

Like an eye
in the cell!

What would you use SPAchip® technology for?

Tell us and help understanding and developing our product