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SPAchip® Technology

Imagine having a lab inside a living single cell with the capacity to make any analysis in an instance and over a long periods of time. Now this is possible with SPAchip technology.

  One of the technological challenges we face nowadays is to fully understand the critical role

of metabolism in a wide variety of diseases

We offer the possibility to analyze several interactions among biomolecules

at real time and capability to track cell process over long time. 

Our SPAchip® technology is set to become biomedicine’s new tool to surveille and

monitor disease progression, individually assess the new drug effect during screening studies and to become the future standard in personalized medicine.

SPAchip® is the perfect tool for cell sensing

by dynamic film about cell feeling

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What do we offer?

Advanced technology in living single cell

A4Cell offers innovative cellomic reagents, named CytoCHECK SPAchip® Detection Kits that enhance the value of in-vitro cell physiology assays used in Cell Imaging analysis.

In studies of diseases such as cancer, it’s very important to improve the metabolic cell studies in order to solve the big questions existing in the progress of the diseases. For that, new live cell imaging techniques is fundamental to enhance the metabolic cell based assays yield.

Analysis of cellular processes for extended periods in real time

the future

A4Cell’s SPAchip® nanodevice is based on the use of inert Silicon microchips on which multiple highly concentrated fluorescent probes can be deposited to provide intracellular readouts over long culture periods following endocytosis.

First device in the market

for multiplexed living single cell analysis


A4Cell is going to optimize its SPAchip© technology to offer high

added value to HCA l HCS and Cell imaging platforms

SPAchip® is the first intracellular device that can be multiplexed with probes emitting in the same wavelength. Advances in surface functionalization techniques allows SPAchip® to be printed with varied molecules (from nucleic acids to proteins, carbohydrates, lipids, or synthetic chemical probes).


Print Procedure 

SPAchip® works detecting intracellular changes for extended period of time.

( estimate of more than one month )


Thanks to a miniaturization process, A4Cell has obtained a device that can be

easily internalized in living cells without affecting the cell viability

or inducing physiological changes


Real images from image analyzer.

A4Cell develop specific kits depending on market demand and new trends in pharmaceutical and biotechnological research

SPAchip® offers the possibility to perform living single cell assays as:

• More sensitive by locally concentrating the fluorescent probe on the chip surface rather than in solution.

• Less toxic by reducing the total cytoplasmic concentration of fluorescent probes.

• Longer duration by fixing the probes on the chip and protecting them from dilution, excretion, or degradation.

• Highly discriminant of cytoplasmic readouts vs signals from other cell compartments.

• More informative by combining various probes on one chip simultaneously.

• More efficient by discriminating signals based on their relative position on the chip rather than their wavelength.

A4cell is delighted to present you a disruptive technology able to

analyze living single cells for extended periods offering innovative

assays with huge advantages:






Joining SPAchip® 

on development applications



SPAchip® technology does not expect to compete with other existing technologies. It opens the door to establish a new and more complete study of the living cell physiopathology with potential applications in academic research but also in drug discovery fields for Pharma and Biotech industry.

Successfully tested, CytoCHECK SPAchip® Detection Kit can be used in combination with:


High-content screening (HCS) Platforms

Are systems uses living cells as tools in biological research to elucidate the workings of normal and

diseased cells. These systems are a combination of modern cell biology, with all their molecular tools, with

automated high-resolution microscopy and robotic handling


Confocal Microscope

In this category the most frequent are confocal laser scanning (LCSM).

This is an optical imaging technique

for increasing optical resolution and contrast of a micrograph.


Cell Imaging Readers

Combines automated digital microscopy and conventional microplate detection in a configurable,

upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield,

high contrast brightfield, color brightfield and phase contrast for maximum applications reach.


Live Cell Metabolic Assay Platforms

The Platform analyzes and measures metabolic functions of live cells with

same-day results. From routine analysis to cutting-edge research

Flow Cytometry Systems

A technique used to detect and measure physical and

chemical characteristics of a population of cells or particles.


Among others...

CytoCHECK SPAchip® 

Lab-in-a-cell assays based on SPAchip® technology


Calcium and pH Detection Kit

ROS Detection Kit

02 Detection Kit

Gene Expression Detection Kit

Customized kit

And much more coming …

Check our deal!
Picked from Calcium and pH

What would you use SPAchip® technology for?


Tell us and help understanding and developing our product, contact us!

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