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SPAchip® Technology Concept

Today, we face a technological challenge: understanding metabolism's critical role in disease . Multiple biomolecule interactions can be analyzed in real time and cell processes can be tracked over time.

Imagine a lab inside a living single cell capable of performing any analysis instantly and over time. SPAchip® technology makes this possible.

A4cell has seamlessly integrated microelectronic and cell biology expertise to develop a comprehensive solution for metabolic cell studies. Our cutting-edge technology enables researchers to effectively monitor and analyze cell behavior during disease progression. With our scientific approach, we strive to empower researchers with the insights they need to make significant strides in the field of cellular biology.

SPAchip® is the perfect tool for cell sensing

by dynamic film about cell feeling

what we offer menu
State of the art

Advanced technology in living single cell

A4cell provides cutting-edge tools and technologies to enhance researchers' capabilities. Staying current is essential for a trusted scientist. We provide them with the latest tools and resources to support their work, encourage innovation, and advance the life sciences industry. Let's push science forward.

Introducing SPAchip® technology - a revolutionary solution for monitoring cellular pathways in real-time! Our intracellular silicon microchips are designed to be easily internalized in living cells without inducing physiological changes or affecting cell viability.

Thanks to a cutting-edge miniaturization process, the SPAchips offer a highly precise and accurate way to analyse intracellular processes with multiple fluorescent probes. By simply changing the SPAchip® fluorescence, you can gain valuable insights into cellular activity. With the SPAchip® kit, you can save time and costs by using it with High Content Screening platforms and imaging analysers. It offers a user-friendly, yet powerful solution for monitoring cellular pathways that will help you take your research to the next level.


So why wait? Try SPAchip® technology today and see the difference it can make for your research!


Real-time monitoring of cellular processes

In the study of diseases such as cancer, it's important to improve metabolic cell studies to answer the big questions. Live cell imaging techniques such as SPAchip® are fundamental to enhancing metabolic cell based assays.

the future

First device in the market

for multiplexed living single cell analysis


A4cell is going to optimize its SPAchip© technology to offer high

added value to HCA l HCS and cell imaging platforms

The SPAchip® is the first intracellular device that can be multiplexed with probes emitting in the same wavelength. Surface functionalization techniques enable SPAchip® to be printed with nucleic acids, proteins, carbohydrates, lipids, or synthetic chemicals for tracking cell metabolism.


Print Procedure 

In response to market demand and emerging trends in pharmaceutical and biotechnology research, A4Cell develops a wide range of kits


Thanks to a miniaturization process, A4Cell has obtained a device that can be

easily internalized in living cells without affecting the cell viability

or inducing physiological changes


Real images from image analyzer.

A4cell develop specific kits depending on market demand and new trends in pharmaceutical and biotechnological research

SPAchip® offers the possibility to perform living single cell assays as:

• More sensitive by locally concentrating the fluorescent probe on the chip surface rather than in solution.

• Less toxic by reducing the total cytoplasmic concentration of fluorescent probes.

• Longer duration by fixing the probes on the chip and protecting them from dilution, excretion, or degradation.

• Highly discriminant of cytoplasmic readouts vs signals from other cell compartments.

• More informative by combining various probes on one chip simultaneously.

• More efficient by discriminating signals based on their relative position on the chip rather than their wavelength.

A4cell is delighted to present you a disruptive technology able to

analyze living single cells for extended periods offering innovative

assays with huge advantages:






Joining SPAchip® 

on development applications



SPAchip® technology does not expect to compete with other existing technologies. It opens the door to establish a new and more complete study of the living cell physiopathology with potential applications in academic research but also in drug discovery fields for Pharma and Biotech industry.

Successfully tested, CytoCHECK SPAchip® Detection Kit can be used in combination with:


High-content screening (HCS) Platforms

Are systems uses living cells as tools in biological research to elucidate the workings of normal and

diseased cells. These systems are a combination of modern cell biology, with all their molecular tools, with

automated high-resolution microscopy and robotic handling


Confocal Microscope

In this category the most frequent are confocal laser scanning (LCSM).

This is an optical imaging technique

for increasing optical resolution and contrast of a micrograph.


Cell Imaging Readers

Combines automated digital microscopy and conventional microplate detection in a configurable,

upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield,

high contrast brightfield, color brightfield and phase contrast for maximum applications reach.


Live Cell Metabolic Assay Platforms

The Platform analyzes and measures metabolic functions of live cells with

same-day results. From routine analysis to cutting-edge research

Flow Cytometry Systems

A technique used to detect and measure physical and

chemical characteristics of a population of cells or particles.


Among others...

What would you use SPAchip® technology for?


Tell us and help understanding and developing our product, contact us!

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