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CytoCHECK SPAchip® pH red single detection kit
CytoCHECK SPAchip® pH red single detection kit
Ref. S-001-PHR
CytoCHEK SPAchip® assay kits are novel fluorescence cell- based assays developed by A4cell that brings together the fields of nanotechnology and cell biology. SPAchips are composed of fluorescently labeled silicon microparticles which can be internalized in the cytosol of cultured cells to monitor intracellular parameters for extended periods of time.t
CytoCHECK SPAchip® pH RED Single-Detection Kit allows measurement of intracellular pH levels by changes in fluorescence intensity, which allows a more comprehensive study of the living single-cell physiology and maximizes the performance of most of imaging analyzers. Its signal in red makes it feasible for applications with high green autofluorescence such as organoids cultures. Moreover, due to its ratiometric behaviour signal to noise ratio is increased leading to cleaner curves.
Kit box contains:
1 ASSAY SPAchip® eppendorf tube (Quantity: 2.5 x10^6 ASSAY SPAchip®)
1 Control SPAchip® eppendorf tube (Quantity: 2.5 x10^5 CONTROL SPAchip®)
1 buffer (Quantity: 5mL)
Quick start guide
Brochure
PRODUCT Sheet
User protocol
Key features
- Measures intracellular pH levels by changes in fluorescence intensity.
- Composed of fluorescently labeled silicon microparticles that can be internalized in the cytosol of cultured cells.
- Provides a more comprehensive study of single-cell physiology and metabolism.
- Maximizes the performance of most imaging analyzers.
- Non-invasive and allows long-term monitoring of intracellular pH changes.
Technical specifications
| Amount | ~2.5 millions of SPAchips |
| Applications | Cell Viability, proliferation, cell image acquisition |
| Assay Time | 30 minutes |
| Assay type | Living single-cell based |
| Detection method | Red Fluorescence |
| Fluorescence | λex: 546 nm; λem: 610 and 707 nm. |
| For research use only | Not for use in diagnostic procedures |
| Incubation time | Overnight |
| Measuring range | pH 4.5 – 9.0 |
| Platform | Fluorescence microscopy, HCS/HCA platforms and flow cytometry |
| Sample type | Adherent cells, suspension cells |
| Shelf Life | 6 months upon arrival |
| Shipping | Environmental conditions |
| Solubility | Soluble in assay buffer (aqueous) |
| State | Solid-phase film with embedded SPAchips |
| Storage Conditions | Refrigerate upon arrival (2ºC to 8ºC) and protect from light |
Like an eye
in the cell!
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