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CytoCHECK SPAchip® pH green single detection kit
CytoCHECK SPAchip® pH green
single detection kit
Ref. S-001-PHG
CytoCHEK SPAchip® assay kits are novel fluorescence cell- based assays developed by A4cell that brings together the fields of nanotechnology and cell biology. SPAchips are composed of fluorescently labeled silicon microparticles which can be internalized inside single cells for lengthy periods, providing highly accurate data through long-term cell monitoring.
CytoCHECK SPAchip ® pH Single-Detection Kit allows measurement of intracellular pH levels by changes in fluorescence intensity, which allows a more comprehensive study of the living single-cell physiology and maximizes the performance of most of imaging analyzers.
Kit box contains:
1 ASSAY SPAchip® eppendorf tube (Quantity: 2.5 x10^6 ASSAY SPAchip®)
1 Control SPAchip® eppendorf tube (Quantity: 2.5 x10^5 CONTROL SPAchip®)
1 buffer (Quantity: 5mL)
Quick start guide
Brochure
Data Sheet
Application note
User protocol
Key features
- Measures intracellular and extracellular pH levels by changes in fluorescence intensity.
- Composed of fluorescently labeled silicon microparticles that can be internalized in the cytosol of cultured cells.
- Provides a more comprehensive study of single-cell physiology and metabolism.
- Non-invasive and allows long-term monitoring of intracellular pH changes.
Technical specifications
| Amount | ~2.5 millions of SPAchips |
| Applications | Cell viability, metabolism, culture CQ |
| Assay type | Living single-cell based |
| Detection method | Changes in green fluorescent intensity |
| Fluorescence | λex:488 nm; λem: 520 nm. |
| For research use only | Not for use in diagnostic procedures |
| Incubation time | Overnight |
| Measuring range | pH 4.5 – 7.5 |
| Platform | Fluorescence microscopy, HCS/HCA platforms and flow cytometry |
| Sample type | Adherent cells, suspension cells |
| Shelf Life | 6 months upon arrival |
| Shipping | Environmental conditions |
| Solubility | Soluble in assay buffer (aqueous) |
| State | Solid-phase film with embedded SPAchips |
| Storage Conditions | Refrigerate upon arrival (2ºC to 8ºC) and protect from light |
Like an eye
in the cell!
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