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CytoCHECK SPAchip® Calcium and pH Multi-Detection Kit
CytoCHECK SPAchip® Calcium and pH Multi-Detection Kit
Ref. M-001-PC
CytoCHECK SPAchip® Calcium and pH Multi-Detection Kit allows continuous, simultaneous, and precise monitoring and measuring of both cytosolic Calcium and intracellular and extracellular pH levels by changes in fluorescence intensity. This facilitates a more comprehensive study of living single-cell physiology. The main advantage of this product is that combines the two pH and calcium detection technologies in one single SPAchip, enabling real-time, simultaneous, and accurate monitoring of intracellular and extracellular pH and calcium levels in single living cells. These cell-based assays are useful for studying and tracking important biological processes as well as for evaluating anti-cancer drug treatments.
CytoCHECK SPAchip® Multi-Detection kits are optimized for its use with confocal microscopes and HCS/HCA analyzers with 20X or over magnification objectives, yet widefield fluorescence microscopes and imaging system with fixed wavelength filters can be also used. Flow cytometers have additionally been validated to analyze intra and extracellular SPAchips.
SPAchip® kits can be handled as common fluorophores and chemical probes for cell biology. After resuspending them in the assay buffer (a SPAchip®-to-cell ratio of 2:1 is recommended), an overnight incubation is required to allow SPAchip® to be incorporated in the cytosol. SPAchip® will remain in the cytosol for longer than one month to monitor cell culture progression.
Kit box contains:
1 ASSAY SPAchip® eppendorf tube (Quantity: 2.5 x10^6 ASSAY SPAchip®)
1 Control SPAchip® eppendorf tube (Quantity: 2.5 x10^5 CONTROL SPAchip®)
1 ASSAY buffer tube (Quantity: 5mL)
Quick start guide
Data Sheet
Application note
User protocol
Key features
- SPAchip® assay kits are novel cell-based assays for living single-cell that bring together the fields of nanotechnology and cell biology.
- CytoCHECK SPAchip® Calcium and pH Multi-Detection Kit enables continuous, simultaneous, and accurate monitoring of intracellular and extracellular pH and Calcium levels in living cells, enabling a more comprehensive study of cell health and physiology.
- CytoCHECK SPAchip® Calcium and pH Multi-Detection kits are composed of fluorescently labeled silicon microparticles -SPAchips®- that can be internalized in cultured cells to monitor changes in specific intracellular analyte concentrations for long periods of time.
- 2-sensors-in-one SPAchip: the main advantage of this product is the combination of our two pH and calcium detection technologies in a single SPAchip device, simplifying cell-based assays by using one single technique. Experimental readouts obtained from the same single cell diminish variability and allow to establish more reliable correlations.
Figure 1: A) Fluorescence signal of SPAchips in an imaging plate with cell culture medium. B) Representative image of SH-SY5Y cells with internalized multiplex SPAchips excitating at 488 nm and emitting at 520 nm. C) Representative image of SH-SY5Y cells with internalized multiplex SPAchips excitating at 546 nm and collecting emission at 610 and 707 nm.
Technical specifications
|
Product code |
M-001-PC | |
|
Amount |
~2.5 millions of SPAchips | |
|
Applications |
Cell viability, proliferation, cell image acquisition | |
|
Assay time |
30 minutes | |
|
Assay type |
Living single-cell based | |
|
Solubility |
Soluble in assay buffer (aqueous) | |
|
Analyte |
Calcium |
pH |
|
Detection method |
Green |
Red fluorescence / Ratiometric curve* |
|
Fluorescence |
λex: 488 nm; λem: 520 nm |
λex: 546 nm; λem: 610 and 707 nm |
|
Measuring range |
10 -1000 μM |
4.5 – 9.0 |
|
Compatible Platforms |
Fluorescence microscopy, HCS/HCA platforms (20x magnification and over) and flow cytometry | |
|
Sample type |
Adherent cells, suspension cells | |
Like an eye
in the cell!
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