SPAchip® Technology Concept
A new world of possibilities: nanotechnology in lab analysis. An extremely precise tool to study the living single cell behavior thanks to our SPAchip® technology. One of the technological challenges we face nowadays is to fully understand the cell physiology.
We offer the possibility to analyze several interactions among biomolecules
at real time and capability to track cell process over long time.
Our SPAchip® technology is set to become biomedicine’s new tool to surveille and
monitor disease progression, individually assess the new drug effect during screening studies and to become the future standard in personalized medicine.
SPAchip® is the perfect tool for cell sensing
Dynamic film about cell feeling
What do we offer?
Advanced technology in living single cell
A4Cell’s primary goal is to develop innovative cellomic reagents, named CytoCHECK SPAchip® Detection Kit, that enhance the value of in-vitro cell physiology assays used in Cell Imaging analyzers and High Content Screening (HCS) platforms.
Analysis of cellular processes for extended periods in real time
A4Cell’s SPAchip® nanodevice is based on the use of inert Silicon microchips on which multiple highly concentrated fluorescent probes can be deposited to provide intracellular readouts over long culture periods following endocytosis.
First device in the market
for multiplexed living single cell analysis
A4Cell is going to optimize its SPAchip© technology to offer high
added value to HCA l HCS and Cell imaging platforms
SPAchip® is the first intracellular device that can be multiplexed with probes emitting in the same wavelength. Advances in surface functionalization techniques allows SPAchip® to be printed with varied molecules (from nucleic acids to proteins, carbohydrates, lipids, or synthetic chemical probes).
Print Procedure - PPL
SPAchip® works detecting intracellular changes for extended period of time.
( estimate of more than one month )
Thanks to a miniaturization process, A4Cell has obtained a device that can be
easily internalized in living cells without affecting the cell viability
or inducing physiological changes
Real images from image analyzer.
A4Cell develop specific kits depending on market demand and new trends in pharmacologic research
SPAchip® offer the possibility to perform living single cell assays as:
• More sensitive by locally concentrating the fluorescent probe on the chip surface rather than in solution.
• Less toxic by reducing the total cytoplasmic concentration of fluorescent probes.
• Longer duration by fixing the probes on the chip and protecting them from dilution, excretion, or degradation.
• Highly discriminant of cytoplasmic readouts vs signals from other cell compartments.
• More informative by combining various probes on one chip simultaneously.
• More efficient by discriminating signals based on their relative position on the chip rather than their wavelength.
A4cell is delighted to present you a disruptive technology able to
analyze living single cells for extended periods offering innovative
assays with huge advantages:
LACK OF TOXICITY
MULTIPLE ANALYSIS POSSIBILITIES
ONE WAVELENGHT FITS ALL!
on development applications
SPAchip® technology does not expect to compete with other existing technologies. It opens the door to establish a new and more complete study of the living cell physiopathology with potential applications in academic research but also in drug discovery fields for Pharma and Biotech industry.
Successfully tested, CytoCHECK SPAchip® Detection Kit can be used in combination with:
High-content screening (HCS) Platforms
Are systems uses living cells as tools in biological research to elucidate the workings of normal and
diseased cells. These systems are a combination of modern cell biology, with all their molecular tools, with
automated high-resolution microscopy and robotic handling
In this category the most frequent are confocal laser scanning (LCSM).
This is an optical imaging technique
for increasing optical resolution and contrast of a micrograph.
Cell Imaging Readers
Combines automated digital microscopy and conventional microplate detection in a configurable,
upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield,
high contrast brightfield, color brightfield and phase contrast for maximum applications reach.
Live Cell Metabolic Assay Platforms
The Platform analyzes and measures metabolic functions of live cells with
same-day results. From routine analysis to cutting-edge research
Flow Cytometry Systems
A technique used to detect and measure physical and
chemical characteristics of a population of cells or particles.
Upcoming assays based on SPAchip® technology
Calcium and pH Detection Kit
ROS Detection Kit
Senescence Detection Kit
Gene Expression Detection Kit
And much more coming …
What would you use SPAchip® technology for?
Tell us and help understanding and developing
our product, contact us!