PRODUCTS
CytoCHECK SPAchip® assay kits are novel fluorescence assays developed by A4Cell that brings together the fields of nanotechnology and cell biology. CytoCHECK SPAchip™ are composed of fluorescently labeled silicon microparticles -SPAchips®- that can be internalized in the cytosol of cultured cells and monitor changes for long periods of time.
CytoCHECK SPAchip® kits are optimized for its use with microscopes and HCS/HCA analyzers with confocal capability and 40X or over magnification objectives, yet epi-fluorescence microscopes and imaging system with fixed wavelength filters can be also used. SPAchips® are multiplexed with an array of arranged fluorescent spots that respond to different stimuli and whose fluorescence can be easily visualized and measured. Depending on the kit, wavelengths and probes will vary.
SPAchips are used as common fluorophores and chemical probes for cell biology. After resuspending them in the assay buffer, simply add 1 µl per well to the cells in a 96-well plate and incubate overnight to let the chips being incorporated in the cytosol. SPAchips® will remain in the cytosol for longer than one month to monitor the evolution of the culture.
The kits based on SPAchip® technology offer cell sensingby
dynamic film about cell feeling
Instructions for Use
CytoCHECK SPAchip®
pH
Single-Detection Kit
pH Single-Detection Kit allows measurement of intracellular pH levels by changes in fluorescence intensity, which allows a more comprehensive study of the living single-cell physiology and maximizes the performance of most of imaging analyzers.
Product Reference: S-001-PHG
For research use only (RUO)
Instructions for Use
CytoCHECK SPAchip® Calcium
Single-Detection Kit
Calcium Single-Detection Kit allows measurement of intracellular Calcium levels by changes in fluorescence intensity, which allows a more comprehensive study of the living single-cell physiology and maximizes the performance of most of imaging analyzers.
Product Reference: S-002-CAG
For research use only (RUO)